Three-dimensional characteristics of T cells and vasculature in the development of mouse esophageal cancer

Three-dimensional characteristics of T cells and vasculature in the development of mouse esophageal cancer

Blog Article

Summary: Esophageal squamous cell carcinoma (ESCC) is a common malignancy, characterized by a multistep pathogenic process regulated spatiotemporally within the esophageal epithelial microenvironment, including vessel normalization and immune infiltration.However, empirical evidence elucidating esophageal vascular remodeling and immune 9.5-4 igora vibrance infiltration during ESCC tumorigenesis in situ is lacking.In this study, utilizing a mouse model recapitulating progressive human ESCC stages, we established a tissue clearing workflow for three-dimensional visualization and analysis of esophageal vessels and T cell distribution.Through this workflow, we delineated the spatial dynamics of vascular remodeling, CD3+ T cells, and characteristic T cell aggregates employing high-resolution light-sheet fluorescence microscopy across five ESCC pathogenic stages.Vessel remodeling might be coupled with T cell infiltration, and annie brocoli dvd their interactions predominantly occurred at the inflammatory stage.

These findings provided insights into research methodologies of esophageal cancer and spatiotemporal landscapes of vascular and T cell during ESCC initiation and progression.